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KMID : 0380319920500000183
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Journal of Korean Research Institute for Better Living
1992 Volume.50 No. 0 p.183 ~ p.193
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Expression and Substrate Specific of UDP-Glucuronosyltransferase lsoenzyme by Steroid Hormone
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Abstract
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The cDNA encoding a member of a family of steroid-UDP-glucuronosyltransferases has been cloned and sequenced. The form of UDP-glucuronosyltransferase designed UDPGT_r-5 is one of a number of closely related species encoded by member of multigene family. In this work northern analysis of a new member of this steroid glucuronidating family of UDP-glucuronosyltransferases is revealed by expression of synthetic oligonucleotide of UDPGT_r-5, 45 specific oligomer and 18 common oligomer nulceotides. Liver RNAs were separated from AD, PB, retinyl acetate, and insulin treated rats. UDPGT_r-5 oligomer hybridization and RNAs were detected by autoradiography. In UDPGT_r-5-specific oligomer hybridization, the 2.3Kb species were active towards androstenedione and retinyl acetate, while UDPGT_r-5 common oligomer active toward retinyl acetate and phenobarbital. Insulin induced RNA was no change 2.3Kb and 3.5Kb in UDPGT_r-5. This result suggested that the expression of UDPGT_r by insulin is controlled at the post translational level. These data showed that UDPGT_r-5 are androstenedione and retinyl acetate UDP-glucuronosyltranferse that is encoded by two mRNAs transcripted from a single gene.
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